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96 well plates  (Beyotime)


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    Beyotime 96 well plates
    The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of <t>a</t> <t>96-well</t> plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images
    96 Well Plates, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/beyogold+96/BeyoGold+96-Well+Black+Opaque+Plates/pmc13077891-125-14-22
    Average 93 stars, based on 36 article reviews
    96 well plates - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types"

    Article Title: A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types

    Journal: Cancer Cell International

    doi: 10.1186/s12935-026-04250-1

    The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images
    Figure Legend Snippet: The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images

    Techniques Used: Suspension, Transferring, Injection

    Related Articles

    Stable Transfection:

    Article Title: Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings
    Article Snippet: Four micrograms of RNA from each group were used for library preparation via the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s instructions and sequenced on an Illumina HiSeq platform. .. A total of 5 × 10 4 CCL-141 cells stably expressing luciferase were subcultured into BeyoGold 96-well black opaque plates (FCP966, Beyotime). .. After replacing the 100 μl of fresh medium, another 100 μl of One-Lumi TM II Firefly Luciferase Reporter Gene Assay Solution (RG056, Beyotime) was added for 5 min. An ELx808 Absorbance Reader (Lonza, Basel, Kanton Basel, Switzerland) was used to detect the signal fluorescein at a wavelength of 570 nm.

    Expressing:

    Article Title: Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings
    Article Snippet: Four micrograms of RNA from each group were used for library preparation via the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s instructions and sequenced on an Illumina HiSeq platform. .. A total of 5 × 10 4 CCL-141 cells stably expressing luciferase were subcultured into BeyoGold 96-well black opaque plates (FCP966, Beyotime). .. After replacing the 100 μl of fresh medium, another 100 μl of One-Lumi TM II Firefly Luciferase Reporter Gene Assay Solution (RG056, Beyotime) was added for 5 min. An ELx808 Absorbance Reader (Lonza, Basel, Kanton Basel, Switzerland) was used to detect the signal fluorescein at a wavelength of 570 nm.

    Luciferase:

    Article Title: Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings
    Article Snippet: Four micrograms of RNA from each group were used for library preparation via the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s instructions and sequenced on an Illumina HiSeq platform. .. A total of 5 × 10 4 CCL-141 cells stably expressing luciferase were subcultured into BeyoGold 96-well black opaque plates (FCP966, Beyotime). .. After replacing the 100 μl of fresh medium, another 100 μl of One-Lumi TM II Firefly Luciferase Reporter Gene Assay Solution (RG056, Beyotime) was added for 5 min. An ELx808 Absorbance Reader (Lonza, Basel, Kanton Basel, Switzerland) was used to detect the signal fluorescein at a wavelength of 570 nm.

    Reporter Gene Assay:

    Article Title: Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings.
    Article Snippet: Four micrograms of RNA from each group were used for library preparation via the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s instructions and sequenced on an Illumina HiSeq platform. .. Firefly luciferase reporter gene assay Atotal of 5×104CCL-141 cells stably expressing luciferasewere subcultured into BeyoGold 96-well black opaque plates (FCP966, Beyotime). .. After replacing the 100 μl of fresh medium, another 100 μl of One-LumiTM II Firefly Luciferase Reporter Gene Assay Solution (RG056, Beyotime) was added for 5min.



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    Image Search Results


    The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images

    Journal: Cancer Cell International

    Article Title: A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types

    doi: 10.1186/s12935-026-04250-1

    Figure Lengend Snippet: The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images

    Article Snippet: In 2D culture, 4,000 Huh7 and 2,000 HLF cells per well were seeded in 96-well plates (BeyoGoldTM black 96-well plates, Cat# FCP966-80pcs, Beyotime).

    Techniques: Suspension, Transferring, Injection